Hypothalamikus struktúrák glutamáterg innervációjának neurokémiai és funkcionális elemzése
Title in English
Glutamatergic innervation of hypothalamic structures: neurochemical and functional analysis
Panel
Neurosciences
Department or equivalent
Neuromorphological and Neuroendocrine Research Laboratory (Office for Research Groups Attached to Universities and Other Institutions)
Participants
Csáki, Ágnes Kiss, József Nagy M., György
Starting date
2003-01-01
Closing date
2006-12-31
Funding (in million HUF)
7.520
FTE (full time equivalent)
0.00
state
closed project
Final report
Results in Hungarian
A [3H]D-aszpartát szelektív felvételén és retrográd transzportján alapuló autoradiográfiás eljárással feltérképeztük a mediális preoptikus areába projiciáló glutamáterg idegsejtek lokalizációját. Leírtuk, hogy vezikuláris glutamát transzporter immunreaktív idegvégződések, melyeket specifikus glutamáterg elemeknek lehet tekinteni, aszimmetrikus szinapszissal kapcsolódnak gonadotrop hormon-releasing hormon immunpozitív neuronokhoz a mediális preoptikus areában, neuropeptid Y, növekedési hormon-releasing hormon és pro-opiomelanocortin tartalmú idegsejtekhez a n. arcuatusban, szomatosztatinerg neuronokhoz a n. periventricularis anteriorban, valamint GABAerg, vazoaktív intesztinális polipeptidet és arginin-vazopresszint tartalmazó idegsejtekhez a n. suprachiasmaticusban. Kimutattuk, hogy nem-NMDA-receptor antagonista a n. paraventricularis hypothalamiba adva blokkolta a szopási inger valamint a formalin-stressz által kiváltott hypophysis prolaktin felszabadulást.
Results in English
By means of the transmitter specific [3H]D-aspartate retrograde tracing technique we detected the location of glutamatergic neurons projecting to the medial preoptic area. We demonstrated synaptic contacts of the asymmetric type between vesicular glutamate transporter type 2 (selective marker of glutamatergic elements) nerve terminals and various neurons such as gonadotrop hormone-releasing hormone immunoreactive neurons in the medial preoptic area, neuropeptide Y, growth hormone-releasing hormone and pro-opiomelanocortin containing neurons in the arcuate nucleus, somatostatinerg nerve cells in the anterior periventricular nucleus and GABA, vasoactive intestinal polypeptide and arginine-vasopressin immunoreactive neurons in the suprachiasmatic nucleus. We observed that non-NMDA receptor antagonist injected into the hypothalamic paraventricular nucleus blocked the suckling stimulus-induced rise in plasma prolactin and the prolactin response to formalin stress.
Bodnár I; Bánky Zs; Nagy MG; Halász B:: Non-NMDA glutamate receptor antagonist injected into the hypothalamic paraventricular nucleus blocks the suckling stimulus-induced release of prolactin, Brain Res Bull 65:163-168, 2005
Kiss J; Kocsis K; Csáki Á; Halász B:: Evidence for vesicular glutamate transporter synapses onto gonadotropin-releasing hormone and other neurons in the rat medial preoptic area, Eur J Neurosci 18:3267-3278, 2003
Kiss J; Csaba Zs; Csáki Á; Halász B:: Glutamatergic innervation of neuropeptide Y- and pro-opiomelanocortin-containing neurons in the hypothalamic arcuate nucleus of the rat, Eur J Neurosci 21:2111-2119, 2005
Nagy GM; Bodnár I; Bánky Zs; Halász B:: Control of prolactin secretion by excitatory amino acids, Endocrine 28:303-308, 2005
Kiss J; Csaba Zs; Csáki Á; Halász B:: Glutamatergic innervation of growth hormone-releasing hormone-containing neurons in the hypothalamic arcuate nucleus and somatostatin-containing neurons in the anterior periventricular nucleus of the rat, Brain Res Bull 70:278-288, 2006
Kocsis K; Kiss J; Csáki Á; Halász B:: Location of putative glutamatergic neurons projecting to the medial preoptic area of the rat hypothalamus, Brain Res Bull 61:459-468, 2003
Bodnár I; Bánky Zs; Halász B:: Non-NMDA glutamate receptor antagonist injected into the hypothalamic paraventricular nucleus inhibits the prolactin response to formalin stress of male rats, Neurochem Res 31:201-208, 2006
