Functional analysis of the fission yeast sep genes  Page description

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Details of project

  
Identifier
42694
Type K
Principal investigator Sipiczki, Mátyás
Title in Hungarian A hasadó élesztőgomba sep génjeinek funkcionális analízise
Title in English Functional analysis of the fission yeast sep genes
Panel Genetics, Genomics, Bioinformatics and Systems Biology
Department or equivalent Department of Genetics and Applied Microbiology (University of Debrecen)
Participants Antunovics, Zsuzsa
Gálné dr. Miklós, Ida
Szilágyi, Zsolt
Starting date 2003-01-01
Closing date 2006-12-31
Funding (in million HUF) 16.560
FTE (full time equivalent) 0.00
state closed project





 

Final report

  
Results in Hungarian
Az egysejtes Schizosaccharomyces pombe ideális modellszervezet az eukarióta sejtciklus tanulmányozásához. A debreceni laboratórium több mint 30 sejtciklus-gént identifikált ebben a szervezetben. A jelenlegi projektben öt sep gén molekuláris biológiai vizsgálatát végezték el. Korábbi eredményeik alapján már tudták, hogy ezek a gének többféle funkciót is ellátnak a sejtben, beleértve a citokinezist követő sejtszeparáció szabályozását is. A mostani pályázat keretében elért eredmények bebizonyították, hogy a sejtszeparációhoz szükséges fehérjéket kódoló gének kulcsfontosságú regulátora a Sep1p transzkripciós faktor. Feladatát az ace2 szabályázásán keresztül látja el. Az ace2 is transzkripciós faktort kódol. A sep10, sep11 és sep15 gének termékei a Mediator-komplex alegységeiként nagyobb géncsoportok koaktivátorai. Részt vesznek az ace2 által szabályozott gének regulációjában is. A SAGA alegységet kódoló sep9 is része a komplex szabályozó mechanizmusnak.
Results in English
The unicellular Schizosaccharomyces pombe is an ideal model for the investigation of the eukaryotic cell cycle. The Debrecen laboratory has identified over 30 cell cycle genes in this organism. The current project was focused on the molecular biological analysis of five sep genes. Previous work done in Debrecen has indicated that these sep genes perform multiple functions including the regulation of cell separation that follows cytokinesis. The results obtained during the project period demonstrated that the transcription factor Sep1p is a key regulator of the genes that code for proteins necessary for the separation process. It exerts its function through the regulation of ace2, a gene that also codes for a transcription factor. The products of the genes sep10, sep11 and sep15 are subunits of the Mediator complex and act as coactivators of larger subsets of other genes, including those controlled by ace2. sep9 encodes a SAGA subunit also involved in the complex regulatory mechanism.
Full text http://real.mtak.hu/746/
Decision
Yes





 

List of publications

  
Sipiczki, M., Szilagyi, Z., Miklos, I.:: The fork-head.type transcription factor Sep1p plays a central role in the regulation of cell separation in Schizosaccharomyces pombe. 1st Central European Forum for Micro, Acta Microbiol. Hung. 52:142, 2005
Lee, K.M., Miklos, I., Du, H., Watt, S., Szilagyi, Z., Saiz, J.E., Madabhushi, R., Sipiczki, M., Bahler, J., Fischer, R.P.:: Impairment of the TFIIH-associated CDK-activating kinase selectively affects cell cycle-regulated gene expression in fission yeast., Mol. Biol. Cell, 16:2734-2745, 2005
Bourbon H-M, sokan mások, Sipiczki M, sokan mások: A unified noemnclature for protein subunits of Mediator complexes linking transcriptional regulators to RNA polymerase II, Molecular Cell 14: 553-557, 2004
Szilagyi Z; Batta G; Enczi K; Sipiczki M: Characterisation of two novel fork-head gene homologues of Schizosaccharomyces pombe: their involvement in cell cycle and sexual differentiation, Gene 348:101-109, 2005
AlonzoNunez ML ; An H; Mehta S; Petit C; Sipiczki M; del Rey F; Gould KL; Vazquez de Aldana CR: Ace2p controls the expression of genes required for cell separation in Schizosaccharomyces pombe, Mol Biol Cell 16:2003-2017, 2005
Alonzo-Nunez ML; An H; Martin-Cuadrado AB; Petit C; Mehta S; Sipiczki M; del Rey F; Gould K; Vazquez de Aldana CR: Ace2p controls the expression of genes required for cell separation in Schizosaccharomyces pombe., The American Society for Cell Biology 2004 Summer Meeting on Cytokinesis, Burlington, Program p. 50, 2004
Miklos, I., Szilagyi, Z., Benko, Z., Enczi, K., Batta, G., Sipiczki, M., Bahler, J.:: Analysis of transcriptional profiles of S. pombe sep10 and sep15 mutant strains, which encode subunits of the Mediator., Yeast 22:S152, 2005
Sipiczki, M., Enczi, K.:: Roles of the fission yeast cell wall in cell polarisation and division: A combined genetic and morphological approach., International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld-2005). Badajoz (Spain). Book of Abstracts p. 974, 2005
Sipiczki, M.:: Cell separation genes in Schizosaccharomyces pombe., Acta Micropbiol. Immunol. Hung. 53:338, 2006
Batta, G., Nagy, R., Antunovics, Z., Miklos, I., Szilagyi, Z., Bahler, J., Sipiczki, M.:: Transcriptional regulation by mediator subunits in Schizosaccharomyces pombe., Acta Micropbiol. Immunol. Hung. 53:247, 2006
Enczi, K., Yamaguchi, M., Sipiczki, M.:: Morphology transition genes in the dimorphic fission yeast Schizosaccharomyces japonicus., Antonie van Leeuwenhoek (in press), 2007
Duran, A., Cortes, J.C., Konomi, M., Sipiczki, M., Martins, I.M., Munoz, J., Moreno, M.B., Osumi, M., Ribas, J.C.:: Functions of the Bgs family of (1,3)-D-glucan synthase catalitic subunits from Schizosaccharomyces pombe., 2nd FEMS Congress of European Microbiologists, Madrid. W53, 2006
Sipiczki, M., Miklos, I., Szilagyi, Z., Batta, G.:: General transcription regulators (Mediator and SAGA) and fork-head transcription factors in the regulation of cytokinesis in Schizosaccharomyces pombe., 34th Annual Conference on Yeasts. Czechoslovak Society for microbiology, Smolenice, p. 30, 2006
Willer, T., Brandl, M., Sipiczki, M., Strahl, S.:: Protein O-mannosylation is crucial for cell wall integrity, septation and viability in fission yeast, Mol. Microbiol. 57:156-170, 2005
Sipiczki, M.:: Splitting of the fission yeast septum. A review., FEMS Yeast Res (in press), 2007
Miklos, I., Szilagyi, Z., Watt, S., Zilahi, E., Batta, G., Antunovics, Z., Enczi, K., Bähler, J,, Sipiczki, M.:: Genomic expression patterns in cell separation mutants of Schizosaccharomyces pombe defective in genes coding for the Mediator subunits Med8/Sep15 and Med31/Sep10, Mol. Gen. Genomics (in press), 2007



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