The role of hypothalamic integrating centers of energy homeoctasis in cental regulation of reproduction  Page description

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Details of project

  
Identifier
46574
Type K
Principal investigator Liposits, Zsolt
Title in Hungarian Az anyagcsere homeostasist szabályozó hypothalamikus neuronrendszerek szerepe a reprodukció központi vezérlésében
Title in English The role of hypothalamic integrating centers of energy homeoctasis in cental regulation of reproduction
Panel Neurosciences
Department or equivalent Laboratory of Endocrine Neurobiology (Institute of Experimental Medicine)
Participants Fekete, Csaba
Hrabák, András
Hrabovszky, Erik
Túri, Gergely
Varju, Patrícia
Starting date 2004-01-01
Closing date 2007-12-31
Funding (in million HUF) 9.056
FTE (full time equivalent) 0.00
state closed project





 

Final report

  
Results in Hungarian
A táplálkozást és a szaporodást szabályozó hypothalamikus központok működését a periféria felől érkező metabolikus szignálok és a jeleket feldolgozó és továbbító orexigén és anorexigén idegsejt csoportok befolyásolják. Morfológiai, molekuláris biológiai, farmakológiai és elektrofiziológiai módszerek együttes alkalmazásával tanulmányoztuk nevezett neuronális szabályozó rendszerek kapcsolatait és a jelátviteli mechanizmusokat. Figyelmünket az orexigén ghrelin, NPY, AGRP, valamint a CART és alfa-MSH tartalmú neuron rendszerekre és azoknak a reprodukciót szabályozó GnRH, valamint a metabolizmust és adaptációt reguláló TRH és CRH rendszerekkel kialakított kapcsolataikra terjesztettük ki. A perifériás hormonhatások közül az ösztrogén és pajzsmirigy hormonok agyi recepciójával és hatásmechanizmusaival foglalkoztunk. Kísérleti munkáinkról 31 tudományos közleményben adtunk számot, 117.71 összesített impakt értékkel. Eredményeink közül kiemeljük a hypothalamikus neuroszekretoros rendszerek glutamáterg fenotípusának felfedezését, a humán GnRH idegsejtek béta típusú ösztrogén receptoron keresztüli szabályozási lehetőségének felismerését, a metabolizmust szabályozó hypothalamikus rendszerek orexigén és anorexigén bemeneteinek jellemzését és eredtük feltárását, a humán agy táplálkozást szabályozó elsődleges hypothalamikus központjának kémiai és szerkezeti jellemzését, valamint a pajzsmirigy hormon aktiválásában résztvevő hypothalamikus szintű szabályozó mechanizmusok feltárását.
Results in English
Peripheral metabolic signals are processed by orexigenic and anorexigenic neuronal networks of the basal hypothalamus that strongly influence the operation of neuroendocrine centers controlling reproduction and metabolism. By combination of structural functional tools, the networking and signal transduction mechanisms of the interacting neuronal systems have been studied. The scope of the work was focused on the orexigenic ghrelin, NPY, AGRP and the anorexigenic CART and alpha-MSH containing neuronal networks and their relationships established with GnRH, TRH and CRH systems, the known neuroendocrine regulators of reproduction, metabolism and adaptation. Regarding the hormonal effects, we have analyzed the receptors and processing of estrogen and thyroid hormone signals. The experimental results have been published in 31 papers with a cumulative impact value of 117.71. Results to be emphasized include the discovery of the glutamate-ergic phenotype of most hypothalamic neurosecretory systems, the structural proof of estradiol regulation of human GnRH neurons via estrogen receptor beta, the elucidation of the chemical nature and origin of the orexigenic and anorexigenic inputs onto hypothalamic neurosecretory systems regulating metabolism and reproduction, the characterization of the feeding centers of the human brain and finally, the cellular and biochemical mechanisms taking part in the activation and deactivation of thyroid hormones within the basal hypothalamus.
Full text http://real.mtak.hu/1494/
Decision
Yes





 

List of publications

  
Menyhért J, Wittmann G, Hrabovszky E, Szlávik N, Keller É, Tschöp M, Liposits Z, Fekete C: Distribution of ghrelin-immunoreactive neuronal networks in the human hypothalamus., Brain Res. 1125(1):31-6, 2006
Farkas I, Varju P, Szabó E, Hrabovszky E, Okada N, Okada H, Liposits Z: Estrogen enhances expression of the complement C5a receptor and the C5a-agonist evoked calcium influx in hormone secreting neurons of the hypothalamus, Neurochem Int (közlésre elfogadva), 2007
Wittmann G, Liposits Z, Lechan RM, Fekete C.: Medullary adrenergic neurons contribute to the cocaine- and amphetamine-regulated transcript-immunoreactive innervation of thyrotropin-releasing hormone synthesizing neurons in the hypothalamic paraventricular nucleus., Brain Res. 1006(1):1-7,, 2004
Wittmann G, Sarkar S, Hrabovszky E, Liposits Z, Lechan RM, Fekete C.: Galanin- but not galanin-like peptide-containing axon terminals innervate hypophysiotropic TRH-synthesizing neurons in the hypothalamic paraventricular nucleus., Brain Res. 1002(1-2):43-50,, 2004
Wittmann G, Lechan RM, Liposits Z, Fekete C.: Glutamatergic innervation of corticotropin-releasing hormone- and thyrotropin-releasing hormone-synthesizing neurons in the hypothalamic paraventricular nucleus of the ra, Brain Res. 1039: 53-62, 2005
Hrabovszky E, Turi GF, Liposits Z.: Presence of vesicular glutamate transporter-2 in hypophysiotropic somatostatin but not growth hormone-releasing hormone neurons of the male rat., Eur J Neurosci. 21: 2120-2126, 2005
Hrabovszky E, Halász J, Meelis W, Kruk MR, Liposits Z, Haller J.: Neurochemical characterization of hypothalamic neurons involved in attack behavior: Glutamatergic dominance and co-expression of thyrotropin-releasing hormone in a subset, Neuroscience. 133: 657-666, 2005
Wittmann G, Liposits Z, Lechan RM, Fekete C.: Origin of cocaine- and amphetamine-regulated transcript-containing axons innervating hypophysiotropic corticotropin-releasing hormone-synthesizing neurons in the rat, Endocrinology. 146: 2985-2991, 2005
Hrabovszky E, Wittmann G, Turi GF, Liposits Z, Fekete C.: Hypophysiotropic thyrotropin-releasing hormone and corticotropin-releasing hormone neurons of the rat contain vesicular glutamate transporter-2., Endocrinology. 146: 341-347, 2005
Menyhért J, Wittmann G, Hrabovszky E, Keller E, Liposits Z, Fekete C: Interconnection between orexigenic neuropeptide Y- and anorexigenic alpha-melanocyte stimulating hormone-synthesizing neuronal systems of the human hypothalamus., Brain Res 1076: 101-105, 2006
Hrabovszky E, Csapó AK, Kalló I, Wilheim T, Turi GF, Liposits Z: Localization and osmotic regulation of vesicular glutamate transporter-2 in magnocellular neurons of the rat hypothalamus., Neurochem Int 48: 753-761, 2006
Hrabovszky E, Kallo I, Turi GF, May K, Wittmann G, Fekete C, Liposits Z: Expression of vesicular glutamate transporter-2 in gonadotrope and thyrotrope cells of the rat pituitary. Regulation by estrogen and thyroid hormone status., Endocrinology 147: 3818-3825, 2006
Halasz J, Toth M, Kallo I, Liposits Z, Haller J: The activation of prefrontal cortical neurons in aggression-A double labeling study., Behav Brain Res 175: 166-175, 2006
Hrabovszky E, Deli L, Turi G, Kalló I, Liposits Zs.: Glutamatergic innervation of the hypothalamic median eminence and posterior pituitary of the rat, Neuroscience 144: 1383-1392, 2007
Hrabovszky E, Liposits Zs.: Glutamatergic phenotype of hypothalamic neurosecretory systems: a novel aspect of central neuroendocrine regulation, Ideggyógyászati Szemle 60: (3-4) 182-186, 2007
Kalló I, Jekkel C, Hrabovszky E, Juranyi Z, Vida B, Jarasi A, Wilheim T, Harsing LG Jr, Liposits Zs.: Immunohistochemical and in situ hybridization studies on glycine transporter 1 after transient ischemia in the rat forebrain, Neurochem Int (ahead of print), 2007
Kiss J, Halasz B, Csaki A, Liposits Z, Hrabovszky E: Vesicular glutamate transporter 2 protein and mRNA containing neurons in the hypothalamic suprachiasmatic nucleus of the rat, Brain Res Bull 74: (6) 397-405, 2007
Liposits Zsolt, Hrabovszky Erik: Novel aspects of glutamatergic signalling in the neuroendocrine system, Journal of Neuroendocrinology (közlésre elfogadva), 2007
Fekete Cs., Freitas BC, Zeöld A., Wittmann G., Kádár A., Liposits Zs., Christoffolete MA, Singru P., Lechan RM., Bianco AC., Gereben B.: Expression patterns of WSB-1 and USP-33 underlie cell-specific posttranslational control of type 2 deiodinase in the rat brain., Endocrinology 148(10):4865-74., 2007
Fekete Cs., Gereben B., Dolschall M., Harney JW., Dora JM., Bianco AC., Sarkar S., Liposits Zs., Rand W., Emerson C., Kacskovics I., Larsen PR., Lechan RM.: Lipopolysaccharide induces type 2 iodothyronine deiodinase in the mediobasal hypothlamus: Implications for the nonthyroidal illness syndrome, Endocrinology 145: 1649-1655, 2004
Fekete Cs., Wittmann G., Liposits Zs., Lechan RM.: Origin of cocaine- and amphetamine-regulated transcript (CART)-immunoreactive innervation of the hypothalamic paraventricular nucleus, J Comp Neurol 469: 340-350, 2004
Hrabovszky E, Kallo I, Steinhauser A, Merchenthaler I, Coen CW, Petersen SL, Liposits Z.: Estrogen receptor-beta in oxytocin and vasopressin neurons of the rat and human hypothalamus: Immunocytochemical and in situ hybridization studies., J Comp Neurol. 473(3):315-33, 2004
Hrabovszky E, Turi GF, Kallo I, Liposits Z.: Expression of vesicular glutamate transporter-2 in gonadotropin-releasing hormone neurons of the adult male rat., Endocrinology, 145(9):4018-21, 2004
Crochemore C, Lu J, Wu Y, Liposits Z, Sousa N, Holsboer F, Almeida: Direct targeting of hippocampal neurons for apoptosis by glucocorticoids is reversible by mineralocorticoid receptor activation., Mol Psychiatr. 10: 790-798, 2005
Zeöld A, Doleschall M, Haffner C, Capelo L, Menyhért J, Liposits Z, Bianco AC, Kacskovics I, Fekete C, Gereben B: Characterization of the NF-kB responsiveness of the human dio 2 gene., Endocrinology 147: 4419-4429, 2006
Farkas Imre, Varjú Patrícia, Liposits Zsolt: Estrogen modulates potassium currents and expression of the Kv4.2 subunit in GT1-7 cells, Neurochem Int 50: 619-627, 2007
Hrabovszky E, Kallo I, Szlavik N, Keller E, Merchenthaler I, Liposits Zs.: Gonadotropin-releasing hormone neurons express estrogen receptor beta, J Clin Endocrinol Metab 92: 2827-2830, 2007
Menyhért J, Wittmann G, Lechan RM, Keller É, Liposits Z, Fekete Cs.: Cocaine- and amphetamine regulated transcript (CART) is colocalized with the orexigenic NPY and AGRP and absent from the anorexigenic alpha-MSH neurons in the infundibular nucleus of the human hypothalamus, Endocrinology 148: 4276-4281, 2007
Wittmann G, Deli L, Kallo I, Hrabovszky E, Watanabe M, Liposits Z, Fekete Cs.: Distribution of type 1 cannabinoid receptor (CB1)-immunoreactive axons in the mouse hypothalamus, J Comp Neurol 503: (2) 270-279, 2007
Fuzesi T, Wittmann G, Liposits Z, Lechan RM, Fekete C: Contribution of noradrenergic and adrenergic cell groups of the brainstem and agouti-related protein (AGRP)-synthesizing neurons of the arcuate nucleus to neuropeptide-Y innervation of corticotropin-releasing hormone neurons in hypothalamic paraventricular nucleus of the rat, Endocrinology 148: 5442-5450, 2007
Turi GF, Liposits Z, Hrabovszky E: Cholinergic afferents to gonadotropin-releasing hormone neurons of the rat, Neurochem Int (közlésre elfogadva), 2007



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