Role of pro- and antioxidant effects in the endoplasmic retikulum stress and apoptosis  Page description

Help  Print 
Back »
 

Details of project

  
Identifier
48939
Type K
Principal investigator Bánhegyi, Gábor
Title in Hungarian Pro- és antioxidáns hatások szerepe az endoplazmás retikulum eredetű stresszben és apoptózisban
Title in English Role of pro- and antioxidant effects in the endoplasmic retikulum stress and apoptosis
Panel Cellular and Developmental Biology
Department or equivalent Dept. of Molecular Biology (Semmelweis University)
Participants Czegle, Ibolya Anett
Kardon, Tamás Zoltán
Lizák, Beáta
Mandl, József
Margittai, Éva
Starting date 2005-01-01
Closing date 2009-12-31
Funding (in million HUF) 17.546
FTE (full time equivalent) 6.36
state closed project





 

Final report

  
Results in Hungarian
Az endoplazmás retikulum számos környezeti és metabolikus hatás szenzora. A jelátvitel gyakran a luminális redoxon keresztül valósul meg. Munkánk során azonosítottuk a hexóz-6-foszfát dehidrogenázt, mint a luminális piridin nukleotidok redox státuszának meghatározóját több sejttípusban. A luminális redox fontos a tápláltság érzékelésében, valamint a sejt apotózis/autofágia szabályozásában. A luminális redoxot befolyásoló antioxidánsok, hepatotoxinok, hormonanalógok és más környezeti hatások alapvetően befolyásolhatják a sejt életképességét.
Results in English
The endoplasmic reticulum is an important sensor and integrator of environmental and metabolic stimuli. The signaling often involves the changes in luminal redox. We have identified the hexose-6-phosphate dehydrogenase as the main determinant of the redox state of luminal pyridine nucleotides in several cell types. Luminal redox is important in nutrient sensing, and in the regulation of programmed cell death. Antioxidants, hepatotoxins, endocrine disruptors and other environmental agents affecting luminal redox can profoundly alter the viability of the cell.
Full text http://real.mtak.hu/1892/
Decision
Yes





 

List of publications

  
Papp E., Nardai G., Mandl J., Bánhegyi G., Csermely P.: FAD oxidizes the Ero1-PDI electron transfer chain. The role of membrane integrity., Biochem. Biophys. Res. Commun. 338, 938-945, 2005
Margittai É., Bánhegyi G., Nagy G., Kiss A., Mandl J., Schaff Z., Csala M.: Scurvy leads to endoplasmic reticulum stress and apoptosis in the liver of guinea pigs., J. Nutr. 135, 2530-2534, 2005
Nagy G., Kardon T., Wunderlich L., Szarka A., Kiss A., Schaff Zs., Bánhegyi G., Mandl J.: Acetaminophen induces ER dependent signaling in mouse liver., Arch. Biochem. Biophys. 459, 273–279, 2007
Czegle I, Piccirella S, Senesi S, Csala M, Mandl J, Bánhegyi G, Fulceri R, Benedetti A.: Cooperativity between 11β-hydroxysteroid dehydrogenase type 1 and hexose-6-phosphate dehydrogenase is based on a pyridine nucleotide pool in the lumen of the endop, Mol. Cell. Endocrinol. 248, 24-25, 2006
Gamberucci A., Konta L., Colucci A., Giunti R., Magyar É.J., Mandl J., Bánhegyi G., Benedetti A., Csala M.: Green tea flavonols inhibit glucosidase II., Biochem. Pharmacol. 72, 640-646, 2006
Bánhegyi G., Csala M., Benedetti A.: Endoplasmic Reticulum: Topics in Chemical Biology., Wiley Encyclopedia of Chemical Biology (invited paper, revised version), 2006
Révész K., Tüttő A., Margittai É., Bánhegyi G., Mandl J., Csala M.: Glucuronide transport across the endoplasmic reticulum membrane is inhibited by epigallocatechin gallate and other green tea polyphenols., Int. J. Biochem. Cell Biol. 39, 922-930, 2007
Csala M., Marcolongo P., Lizák B., Senesi S., Margittai É., Fulceri R., Magyar É.J., Benedetti A, Bánhegyi G.: Transport and transporters in the endoplasmic reticulum (review), Biochim. Biophys. Acta – Biomembr. 1768, 1325-1341, 2007
Csala M., Margittai É., Senesi S., Gamberucci A., Bánhegyi G., Mandl J., Benedetti A: Inhibition of hepatic glucose 6-phosphatase system by the green tea flavanol epigallocatechin gallate., FEBS Lett. 581, 1693-1698, 2007
Giunti R., Gamberucci A., Fulceri R., Bánhegyi G., Benedetti A.: Both translocon and a cation channel are involved in the passive Ca2+ leak from the endoplasmic reticulum: a mechanistic study on rat liver microsomes., Arch. Biochem. Biophys. 462, 115-121, 2007
Lizák B., Csala M., Benedetti A., Bánhegyi G.: The translocon and the non-specific transport of small molecules in the endoplasmic reticulum., Mol. Membr. Biol. 25, 95-101, 2007
Margittai É., Bánhegyi G.: Isocitrate dehydrogenase: a NADPH generating enzyme in the lumen of the endoplasmic reticulum., Arch. Biochem. Biophys. 471, 184–190, 2008
Kardon T., Senesi S., Marcolongo P., Legeza B., Bánhegyi G., Mandl J., Fulceri R., Benedetti A.: Maintenance of luminal NADPH in the endoplasmic reticulum promotes the survival of human neutrophil granulocytes., FEBS Lett. 582, 1809-1815, 2008
Bánhegyi G., Mandl J., Csala M.: Role of endoplasmic reticulum redox imbalance in neuronal cell death., J. Neurochem. 107, 20-34, 2008
Czegle I., Margittai É., Senesi S., Benedetti A., Bánhegyi G.: Different expression and distribution of 11β-hydroxysteroid dehydrogenase type 1 in obese and lean animal models of type 2 diabetes., Acta Physiol. Hung. 95, 419-424, 2008
Magyar J.É., Gamberucci A., Konta L., Margittai É., Benedetti A., Bánhegyi G., Mandl J., Csala M.: Endoplasmic reticulum stress underlying the pro-apoptotic effect of epigallocatechin gallate in mouse hepatoma cells., Int. J. Biochem. Cell Biol. 41, 694-700, 2009
Csala M., Bánhegyi G., Benedetti A.: Endoplasmic reticulum: A metabolic compartment. (review), FEBS Lett. 580, 2160-2165, 2006
Lizák B., Czegle I., Csala M., Benedetti A., Mandl J., Bánhegyi G.: Translocon Pores in the Endoplasmic Reticulum Are Permeable to Small Anions., Am. J. Physiol. – Cell Physiol. 291, C511-517, 2006
Piccirella S., Czegle I., Lizák B., Margittai É., Senesi S., Papp E., Csala M., Fulceri R., Csermely P., Mandl J., Benedetti A., Bánhegyi G.: Uncoupled redox systems in the lumen of the endoplasmic reticulum: pyridine nucleotides stay reduced in an oxidative environment., J. Biol. Chem. 281, 4671-4677, 2006
Bánhegyi G., Benedetti A., Csala M., Mandl J.: Stress on redox, FEBS Lett. 581, 3634-3640, 2007
Marcolongo P., Piccirella S., Senesi S., Wunderlich L., Gerin I., Mandl J., Fulceri R., Bánhegyi G., Benedetti A.: The glucose-6-phosphate transporter - hexose-6-phosphate dehydrogenase - 11ß-hydroxysteroid dehydrogenase type 1 system of the adipose tissue., Endocrinology 148, 2487-2495, 2007
Margittai É., Löw P., Szarka A., Csala M., Benedetti A., Bánhegyi G.: Intraluminal hydrogen peroxide induces a permeability change of the endoplasmic reticulum membrane., FEBS Lett. 582, 4131-4136, 2008
Senesi S., Marcolongo P., Gava B., Fulceri R., Sorrentino V., Margittai É., Lizák B., Csala M., Bánhegyi G., Benedetti A.: Metyrapone prevents cortisone-induced preadipocyte differentiation by depleting luminal NADPH of the endoplasmic reticulum., Biochem. Pharmacol. 76, 382-390, 2008
Senesi S., Marcolongo P., Manini I., Fulceri R., Sorrentino V., Csala M., Bánhegyi G., Benedetti A.: Constant Expression of Hexose-6-Phosphate Dehydrogenase during Differentiation of Human Adipose-derived Mesenchymal Stem Cells., J. Mol. Endocrinol. 41, 125–133, 2008
Bánhegyi G., Csala M., Benedetti A.: Hexose-6-phosphate dehydrogenase: linking endocrinology and metabolism in the endoplasmic reticulum., J. Mol. Endocrinol. (in press) http://dx.doi.org/10.1677/JME-08-0156, 2009
Mandl J., Mészáros T., Bánhegyi G., Hunyady L., Csala M.: Endoplasmic reticulum: nutrient sensor in physiology and pathology., Trends Endocrinol. Metab. (accepted), 2009



Back »