Morphological analyses of the glutamatergic innervation of cholinergic and GABAergic neuronal populations located in the rat basal forebrain cholinergic system
A bazális előagyi kolinerg és GABAerg neuronpopulációk glutamáterg beidegzésének morfológiai elemzése
Title in English
Morphological analyses of the glutamatergic innervation of cholinergic and GABAergic neuronal populations located in the rat basal forebrain cholinergic system
Panel
Neurosciences
Department or equivalent
Neuromorphological and Neuroendocrine Research Laboratory (Office for Research Groups Attached to Universities and Other Institutions)
Participants
Csaba, Zsolt Csáki, Ágnes Halász, Béla
Starting date
2005-01-01
Closing date
2009-06-30
Funding (in million HUF)
8.580
FTE (full time equivalent)
4.42
state
closed project
Final report
Results in Hungarian
A basal forebrain (BFC) különböző régióiban a VGluT2 megjelenítésével glutamáterg idegrost-hálózatot mutattunk ki, mely aszimmetrikus kapcsolatokat képezett immunjelölt kolinerg, illetve GABAerg idegsejtekkel. Előfordultak VGluT2-immunarany plus ChAT-DAB-immunjelölt idegvégződések is szinaptikus kapcsolatban kolinerg neuronokkal. Glutamáterg szinapszisok nagyobb számban fordultak elő GABAerg mint kolinerg neuronokon, melyek kolinerg sejteken leggyakrabban dendrittörzseken, GABAerg neuronokon proximális dendritszakaszokon fordultak elő. Vizsgáltuk, hogy a BFC capsula internában lévő neuronok részt vesznek-e az entorhinalis kéreg (EC) afferentációban. Az EC-be injektált wheat germ agglutinin-gold retrográd transzport feltárta kolinerg, GABAerg és calretinint tartalmazó sejtek részvételét. [3H]D-aszpartátot alkalmazva kimutattuk, hogy az EC-hez vetítő kolinerg és CR-tartalmú neuronokban glutamát is van. - Megállapítottuk, hogy androgén receptort tartalmazó glutamáterg idegsejtek előfordulnak a septumban, bed nucleusban, hypothalmusban. Közöltük, hogy glutamáterg idegrostok végződnek GHRH neuronokon az arcuatusban, somastostatin tartalmú idegsejteken a nucleus periventricularis anteriorban, és VIP, AVP és GAD-ir neuronokon a nucleus suprachiasmaticusban. Felderítettük a táplálkozás és szexuális magatartás szabályozásában szerepet játszó nucleus ventromedialisban végződő glutamáterg rostok eredő sejtjeinek lokalizációját
Results in English
We studied the glutamatergic innervation of cholinergic and GABAergic neurons in regions of the basal forebrain (BFC). Using vesicular glutamate transporter type2 (VGluT2) as marker of glutamatergic elements, we demonstrated numerous VGluT2 ir axon terminals in synaptic contact of the asymmetric type with cholinergic and GABAergic neurons. VGluT2 immungold plus DAB immunolabelled cholinergic nerve fibers terminating on dendrites of cholinergic neurons were also detected. There were more glutamatergic synapses on GABAergic than on cholinergic neurons. By means of retrograde transport of conjugated wheat germ agglutinin we observed that GABAergic and calretinin containing neurons in the BFC internal capsule project to the entorhinal cortex, and using [3H]D-asparte cholinergic and calretinin containing neurons terminating in the entorhinal cortex contain also glutamate. – Further, we found glutamatergic neurons containing androgen receptor in the lateral septum, bed nucleus and in the hypothalamus. We published that glutamatergic fibres terminate on GHRH neurons in the arcuate, on somatostatin-ir nerve cells in the anterior periventricular and on VIP, AVP and GAD-ir neurons in the suprachiasmatic nucleus. Finally, we revealed by means of [3H]D-aspartate retrograde autoradiography the location of glutamatergic neurons projecting to the hypothalamic ventromedial nucleus involved in the control of feeding and sexual behaviour.
1.) Kiss J; Csaba Zs; Csáki Á; Halász B:: Glutamatergic innervation of neuropeptide Y and of pro-opiomelanocortin-containing neurons in the hypothalamic arcuate nucleus of the rat, European Journal of Neuroscience 21, 2111-2119, 2005
3.) Kiss J; Csaba Zs; Csáki Á; Halász B:: Glutamatergic innervation of growth hormone-releasing hormone-containing neurons in the hypothalamic arcuate nucleus and somatostatin-containing neurons in the [..., Brain Research Bulletin 70, 278-288, 2006
2.) Kiss J; Csaba Zs; Csáki Á; Halász B:: The localization of type 2 vesicular glutamate transporter (VGluT2) and/or vesicular acetylcholine transporter (VAChT) containing nerve fibres in the hippocampus and [..., Clinical Neuroscience 58/1 pp:50-51, 2005
4.) Kiss J; Csáki Á; Csaba Zs; Halász B:: Morphological analysis of glutamatergic innervation of cholinergic and non-cholinergic neuron populations in the basal forebrain. I. Medial septum-diagonl band complex., Clinical Neuroscience 59/1 p.33, 2006
8.) Kiss J; Csáki Á; Halász B:: Glutamatergic afferent inputs to cholinergic and GABAergic neurons located in various subregions of the rat basal forebrain, Clinical Neuroscience 60(1): 32-33., 2007
5.) Csaba Zs; Kiss J; Csáki Á; Halász B:: Comparative distribution of androgn receptor- and vesicular glutamate transporter 2-containing neurons in the septum, hypothalamus and amygdala of the rat, Clinical Neuroscience 60(1): 14-15., 2007
9.) Kiss J; Halász B; Csáki Á; Liposits Z; Hrabovszky E:: Vesicular glutamate transporter 2 protein and mRNA containing neurons in the hypothalamic suprachiasmatic nucleus of the rat, Brain Research Bulletin 74: 397-405., 2007
6.) Csáki Á; Csaba Z; Halász B; Kiss J:: Neurochemical characteristics of basalocortical projecting neurons located in the basal forebrain, and their relationships to glutamatergic innervation, Clinical Neuroscience, in press, 2007
7.) Kiss J; Csáki Á; Csaba Z; Halász B:: Synaptic contacts of vesicular glutamate transporter type 1 and 2 fibres on chemically identified neurons of the hypothalamic suprachiasmatic nucleus of the rat, Eur J of Neuroscience, közlésre beküldve, 2007
10.) Kiss J; Csáki Á; Csaba Zs; Halász B:: Synaptic contacts of vesicular glutamate transporter 2 fibres on chemically identified neurons of the hypothalamic suprachiasmatic nucleus of the rat., European Journal of Neuroscience 28(9):1760-74., 2008
11.) Kiss J; Csáki Á; Csaba Zs; Halász B:: Basalocortical projecting neurons: Neurochemical characteristics and glutamatergic innervation., 6th FENS Forum of European Neuroscience, Geneva 2008 - CD ROM, 2008
12.) Agnes Csaki; Jozsef Kiss; Bela Halasz: Glutamatergic Neurons Projecting to the Hypothalamic Ventromedial Nucleus of the Rat, Frontiers in Systems Neurosciens - Conferenc Anbstract: 12th Meeting of The HNS,, 2009
13.) József Kiss; Ágnes Csáki, Zsolt Csaba, Béla Halasz: Neurochemical characters of basal forebrain corticopetal neurons projecting to entorhinal and prefrontal cortices, Frontiers in Systems Neurosciens conferenc Ansrtact - 12th Meeting of The HNS, 2009
