Artificial receptors based on surface imprinted electrosynthesized polymer nanostructures for protein sensing  Page description

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Details of project

 
Identifier
117637
Type NN
Principal investigator Gyurcsányi, Ervin Róbert
Title in Hungarian Felületi lenyomatú polimer nanoszerkezeteken alapuló szintetikus receptorok fehérjék meghatározásához
Title in English Artificial receptors based on surface imprinted electrosynthesized polymer nanostructures for protein sensing
Keywords in Hungarian kémiai érzékelés, molekuláris lenyomatú polimerek, fehérje felismerés, szintetikus receptorok, felületi plazmon rezonancia
Keywords in English chemical sensing, molecularly imprinted polymer, protein recognition, artificial receptors, surface plasmon resonance
Discipline
Analytical Chemistry (Council of Physical Sciences)100 %
Ortelius classification: Chemical and biosensors
Panel Chemistry 1
Department or equivalent Department of Inorganic and Analytical Chemistry (Budapest University of Technology and Economics)
Participants Erdőssy, Júlia
Horváth, Viola
Jágerszki, Gyula
Starting date 2015-09-01
Closing date 2019-08-31
Funding (in million HUF) 34.356
FTE (full time equivalent) 6.20
state running project





 

Final report

 
Results in Hungarian
A molekuláris lenyomatképzés során egy “sablon” molekula jelenlétében megfelelő funkcionalitású monomereket polimerizálva a sablon molekulára szelektív kötőhelyeket lehet kialakítani. Az egyik legnagyobb kihívás ezen a kutatási területen a fehérjék szelektív felismerésére alkalmas molekuláris lenyomatú polimerek (MIP) előállítása. A kutatási projektben átfogó megoldásokat dolgoztunk ki a fehérje lenyomatképzés sikeres megvalósítására. - Új, elektroszintézisen alapuló módszereket dolgoztunk ki MIP-ek előállítására, amelyek során a fehérje sablon szerkezeti változása elkerülhető. - Elektrokémiai szintézissel sikerült kontrollált módon a fehérjék átmérőjével összemérhető vastagságú MIP nanofilmeket előállítani. Ezeket párosítva az általunk bevezetett elektrokémiai mikrocseppentéses eljárással megvalósítottuk a MIP nanofilmeken alapuló fehérje chipek nagy áteresztőképességű előállítását. Ezeket a MIP nanofilmeket sikeresen alkalmaztuk fehérjék jelölés nélküli meghatározására felületi plazmon rezonancia képalkotással és elektrokémiai detektálással. - Új monomereket és monomer könyvtárakat szintetizáltunk vektoriális lenyomatképzésre és hibrid MIP-ek előállítására. A kutatás során magyarázatot adtunk a MIP-fehérje kölcsönhatásoknál esetenként tapasztalt irreálisan nagy affinitási állandókra és szelektivitási anomáliákra. A projekt során nagyszámú fehérjére állítottunk elő MIP nanofilmeket, amelyek gyakorlati alkalmazhatóságát is sikerült több esetben bizonyítani.
Results in English
Molecular imprinting is a concept to generate materials with “molecular memory” by polymerization of suitable functional monomers in the presence of a target molecule acting as a template. Imprinting polymers with proteins is extremely challenging. This relates mainly with their fragility, as well as the difficulty to avoid permanent entrapment and generation of cross-reactive binding sites. In this project we addressed these fundamental difficulties of protein imprinting. • We introduced novel electrosynthetic methods for the preparation of molecularly imprinted polymers (MIPs) that enable imprinting in mild conditions to avoid target denaturation. • We introduced methods to generate polymer nanofilms with thicknesses comparable with the diameter of the protein targets. These included microelectrospotting for high throughput electrosynthesis of MIP-based protein chips. Such MIP nanofilms were successfully used for affinity assays using either multiplexed surface plasmon resonance imaging or electrochemical detection. • Novel functional monomers and monomer libraries were custom synthesized and implemented in combination with vectorial imprinting and hybrid MIPs. We also identified a number of fundamental artifacts that may lead to unrealistic affinity constants for protein MIP interactions. A large number of protein targets were tested and for some of them (human serum albumin, glycosylated hemoglobin, acetylcholinesterase) the practical applicability is imminent.
Full text https://www.otka-palyazat.hu/download.php?type=zarobeszamolo&projektid=117637
Decision
Yes





 

List of publications

 
Jetzschmann KJ, Jágerszki G, Dechtrirat D, Yarman A, Gajovic-Eichelmann N, Gilsing HD, Schulz B, Gyurcsányi RE, Scheller FW: Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition, Advanced Functional Materials, 2015
Bosserdt M, Erdőssy J, Lautner G, Witt J, Kohler K, Gajovic-Eichelmann N, Yarman A, Wittstock G, Scheller FW, Gyurcsányi RE: Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition, Biosensors and Bioelectronics, 2015
Erdőssy J, Horváth V, Yarman A, Scheller FW, Gyurcsányi RE: Electrosynthesized molecularly imprinted polymers for protein recognition, TrAC- Trends in Analytical Chemistry, 2016
Menger M, Yarman A, Erdőssy J, Yildiz HB, Gyurcsányi RE, Scheller FW: MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing, Biosensors, 2016
He N, Gyurcsányi RE, Lindfors T: Electropolymerized hydrophobic polyazulene as solid-contacts in potassium-selective electrodes, Analyst, 2016
Jetzschmann KJ, Jágerszki G, Dechtrirat D, Yarman A, Gajovic-Eichelmann N, Gilsing HD, Schulz B, Gyurcsányi RE, Scheller FW: Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition, Advanced Functional Materials, 25: 5178–5183, 2015
Bosserdt M, Erdőssy J, Lautner G, Witt J, Kohler K, Gajovic-Eichelmann N, Yarman A, Wittstock G, Scheller FW, Gyurcsányi RE: Microelectrospotting as a new method for electrosynthesis of surface-imprinted polymer microarrays for protein recognition, Biosensors and Bioelectronics, 73, 123-129, 2015
Erdőssy J, Horváth V, Yarman A, Scheller FW, Gyurcsányi RE: Electrosynthesized molecularly imprinted polymers for protein recognition, TrAC- Trends in Analytical Chemistry, 79, 179-190, 2016
Menger M, Yarman A, Erdőssy J, Yildiz HB, Gyurcsányi RE, Scheller FW: MIPs and Aptamers for Recognition of Proteins in Biomimetic Sensing, Biosensors 6(3), 35, 2016
He N, Gyurcsányi RE, Lindfors T: Electropolymerized hydrophobic polyazulene as solid-contacts in potassium-selective electrodes, Analyst, 141, 2990-2997, 2016
Stojanovic Z, Erdőssy J, Keltai K, Scheller FW, Gyurcsányi RE: Electrosynthesized molecularly imprinted polyscopoletin nanofilms for human serum albumin detection, Analytica Chimica Acta, 977, 1-9, 2017
He N, Papp S, Lindfors T, Höfler R, Latonen RM, Gyurcsányi RE: Pre-Polarized Hydrophobic Conducting Polymer Solid-Contact Ion-Selective Electrodes with Improved Potential Reproducibility, Analytical Chemistry 89 (4), 2598–2605, 2017
Scheller FW, Jetzschmann KJ, Zhang X, Yarman A, Wollenberger U, Erdőssy J, Gyurcsányi RE: Electrosynthesized MIPs for Proteins: Plastibodies or Nano-Filters, International Conference on Electrohemical Sensors, 2017
Páncsics MM, Horváth V, Gyurcsányi RE: Fluorescence Anisotropy Measurement to Assess Protein Binding to Nanoparticles, International Conference on Electrohemical Sensors, 2017
Stojanovic Z, Erdőssy J, Keltai K, Scheller FW, Gyurcsányi RE: Electrosynthesized molecularly imprinted polyscopoletin nanofilms for human serum albumin detection, Analytica Chimica Acta, 977, 1-9, 2017
He N, Papp S, Lindfors T, Höfler R, Latonen RM, Gyurcsányi RE: Pre-Polarized Hydrophobic Conducting Polymer Solid-Contact Ion-Selective Electrodes with Improved Potential Reproducibility, Analytical Chemistry 89 (4), 2598–2605, 2017
Zhang Xiaorong; Yarman Aysu; Erdőssy Júlia; Katz Sagie; Zebger Ingo; Jetzschmann Katharina J; Altintas Zeynep; Wollenberger Ulla; Gyurcsányi Ervin Róbert; Scheller Frieder W: Electrosynthesized MIPs for transferrin: Plastibodies or nano-filters?, BIOSENSORS & BIOELECTRONICS, 105, 29-35., 2018
Szakács Z; Mészáros T; De Jonge MI; Gyurcsányi RE: Selective counting and sizing of single virus particles using fluorescent aptamer-based nanoparticle tracking analysis, Nanoscale, 10 (29), 13942-13948., 2018
Gyurcsányi RE; Szakács Z; Stojanovic Z.; Erdőssy J; Mészáros T; Scheller FW: Selective Synthetic Receptors for Proteins: Approaching Practical Applications, Pittcon 2018. 27 Feb - 1 Mar 2018, Orlando, FL, USA, 2018
Zhang Xiaorong; Yarman Aysu; Erdőssy Júlia; Katz Sagie; Zebger Ingo; Jetzschmann Katharina J; Altintas Zeynep; Wollenberger Ulla; Gyurcsányi Ervin Róbert; Scheller Frieder W: Electrosynthesized MIPs for transferrin: Plastibodies or nano-filters?, BIOSENSORS & BIOELECTRONICS, 105, 29-35., 2018
Simon L, Gyurcsányi RE: Multiplexed assessment of the surface density of DNA probes on DNA microarrays by surface plasmon resonance imaging, Analytica Chimica Acta, 1047, 131-138, 2019
Scheller FW, Zhang X, Yarman A, Wollenberger U, Gyurcsányi RE: Molecularly imprinted polymer-based electrochemical sensors for biopolymers, Current Opinion in Electrochemistry, 2019
Jetzschmann KJ, Tank S, Jágerszki G, Gyurcsányi RE, Wollenberger U, Scheller FW: Bio‐Electrosynthesis of Vectorially Imprinted Polymer Nanofilms for Cytochrome P450cam, ChemElectroChem, 6,1818-1823., 2019
Papp S, Bojtár M, Gyurcsányi RE, Lindfors T: Potential Reproducibility of Potassium-Selective Electrodes Having Perfluorinated Alkanoate Side Chain Functionalized Poly(3,4-ethylenedioxytiophene) as a Hydrophobic Solid Contact, Analytical Chemistry, 9, 114, 9111-9118, 2019
Terejánszky P, Papp S, Berényi S, Gyurcsányi RE: Resistive Pulse Sensing as a High-Resolution Nanoparticle Sizing Method: A Comparative Study, Particle & Particle Systems Characterization, 36, 1800543., 2019
Scheller FW, Yarman A, Zhang X, Appelt KJ, Wollenberger U, Caserta G, Zebger I, Lenz O, Ozcelikay G, Kurbanoglu S, Ozkan SA, Gyurcsányi ER: Peptides, Tags and Proteins as Templates in Electrosynthesized Mips, International Conference on Chemical Sensors, 2019
Supala E, Bojtár M, Gyurcsányi ER: Protein Recognition with Molecularly Imprinted Polymer Based on EDOT-Derivatives, International Conference on Chemcal Sensors, Visegrád, June 16-21, 2019
Zhang X, Yarman A, Caserta G, Zebger I, Wollenberger U, Gyurcsányi RE, Scheller FW: Fully Electrochemical Mip for the N-Terminal Peptide of Human Hemoglobin, International Conference on Chemical Sensors, Visegrád, June 16-21, 2019




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