Analyses of factors regulating fish sex using genomic tools  Page description

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Details of project

  
Identifier
140353
Type KKP
Principal investigator Orbán, László
Title in Hungarian Halak ivarát szabályozó tényezők vizsgálata a genomika eszközeivel
Title in English Analyses of factors regulating fish sex using genomic tools
Keywords in Hungarian halszaporodás, ivarmeghatározás, genetikai szabályozás, környezeti hatások, globális felmelegedés
Keywords in English fish reproduction, sex determination, genetic regulation, environmental effects, global warming
Discipline
Livestock genomics (Council of Complex Environmental Sciences)40 %
Pisciculture, fishery (Council of Complex Environmental Sciences)40 %
Animal reproduction biology (Council of Complex Environmental Sciences)20 %
Panel Plant and animal breeding
Department or equivalent Institute for Aquaculture and Environmental Safety (Hungarian University of Agriculture and Life Sciences)
Participants Bársony, Péter
Beliczky, Gábor Péter
Bercsényi, Miklós
Bognár, András
Havasi, Máté
Hirth, Mirella
Horváth, Kornél
Kovács, Balázs
Kovács, Gyula
Maász, Gábor
Majoros, Gábor
Marton, Csaba
Merth, János
Molnár, Kálmán
Székely, Csaba
Szeverényi, Ildikó
Varga, Máté
Zsolnai, Attila
Starting date 2020-08-01
Closing date 2023-12-31
Funding (in million HUF) 138.511
FTE (full time equivalent) 18.54
state running project





 

Final report

  
Results in Hungarian
A projekt fő célja a halak ivarát szabályozó genetikai és környezeti tényezők vizsgálata volt a genomika eszközeivel. Elsősorban a zebradániót használtuk modellként kutatásainkhoz, de más modell és haszon halfajokat is vizsgáltunk. A zebradánió ivarának vizsgálatához két mutáns vonalat használtunk, melyek segítették az ivarszerv kialakulásának jobb megértését. Kifejlesztettünk egy olyan transzgénikus jelzővonalat is, melyben a test csökkent pigmentációja miatt az ivari különbségeket jobban megfigyelhetjük. Egy speciális eljárást is kidolgoztunk, mellyel az ivarszervek kialakulását nagyszámú élő egyeden egyszerre tudtuk követni. Újra megvizsgáltuk a női egyedek hővel végzett átszexálását, hogy tovább optimalizáljuk az eljárást és hőérzékeny családokat azonosítsunk. A genomban kódolt és expresszált ivari markereket azonosítottunk két halfajból. Részt vettünk olyan kutatásokban is, melyek haszonhal fajok szaporodásáról ill. genomjáról bővítették ismereteinket. Sikerrel alkalmaztunk egy hőkezeléses eljárást kopoltyúféreg parazitával fertőzött lesőharcsa egyedek megmentésére. A kezelés jelentősen csökkentette a fertőzött kopoltyúk parazita terhelését a kezeletlen egyedekéhez képest. A hőkezelt és kezeletlen egyedek kopoltyújában és lépében kifejeződő génkészletek összehasonlítása révén több, mint 600 olyan gént azonosítottunk, amelyek expressziójának mértéke statisztikailag szignifikáns különbséget mutatott.
Results in English
This project aimed to achieve better understanding of the genetic and environmental regulators of fish sex using the tools of genomics. We used the zebrafish as a model for our studies, but worked on several other model and food fish species as well. For the analysis of zebrafish sex, we have utilized two mutants, that helped us to gain better understanding of the gonad differentiation of the species. We have developed an improved transgenic reporter line with reduced pigmentation of the body to allow for better detection of differences between the two sexes. We have also worked out a special protocol that allowed us to follow gonad differentiation processes in vivo on an individual basis on a large number of specimens. We have revisited the formerly tested phenomenon of heat-induced masculinization to optimize the procedure and identify heat-sensitive families. We have discovered genomic and expressed sex markers in two fish species. We have taken part in the analysis of several food fish species to deepen our knowledge on the reproduction and/or genome. We have successfully used a heat-based treatment to rescue European catfish individuals infected by a gill monogenean exoparasite. The treatment has substantially reduced the parasite loads on the gills in comparison to untreated, infected controls. Comparative analysis of the heat-treated vs. untreated gills and spleens identified over 600 genes whose expression level changed significantly as a result of the treatment.
Full text https://www.otka-palyazat.hu/download.php?type=zarobeszamolo&projektid=140353
Decision
Yes





 

List of publications

  
Komissarov, A., S. Vij, A. Yurchenko, V. Trifonov, N.M. Thevasagayam, P.S.R. Sridatta, K. Purushothaman, A. Graphodatsky, L. Orbán, I. Kuznetsova:: B chromosomes of the Asian seabass (Lates calcarifer) contribute to genome variations at the level of individuals and populations, Genes 9: 464, 2018
Liew, WC., L. Ribas, N. Díaz, R. Sreenivasan, JM. Saju, K-W. Tzung, R. Goto, T. Saito, E. Yamaha, MS. Hossain, MEK. Calvert, K. Arai, F. Piferrer and L. Orbán: Analysis of regulators of fish sex using the zebrafish model, Oral presentation at LXI. Hungarian Hydrological Society Conference 2019. október 2-4. Tihany, Hungary, 2019
Orbán, L., J.M. Saju, K-W Tzung and WC Liew: Masculinization of zebrafish through partial depletion of primordial germ cells by injecting diluted Morpholino oligonucleotides into embryos, Invited book chapter in the Springer Protocols series on Methods in Molecular Biology (Guest Ed.: Roland Dosch; Series Ed.: J. M Walker) Springer Verlag, Berlin, Germany, 2020
Orbán, L., Liew, W. C., Saju, J., Tzung, K., Bognár, A., Beliczky, G., Marton, Cs., Szeverényi, I.: Zebrafish males are models of natural sex reversal in teleosts, FIBOK; Gödöllő, Hungary, April 12., Abctract Book p. 101, 2022
Orban, L.: Selection program of Asian seabass - made in Singapore, Invited e-talk on ’Regional Capacity Building Program organized by ICAR – National Bureau of Fish Genetic Resources, Lucknow, UP, India, 17th of January, 2022
Balogh, RE, B. Csorbai, Cs. Guti, Sz. Keszte, B. Urbányi, L. Orbán and B. Kovács: Validation of a male-specific DNA marker for molecular sexing in several Hungarian strains of African catfish (Clarias gariepinus), Theriogenology 205: 106-113, 2023
Shen, XY, YC. Niu, JAV. Uichanco, N. Phua, P. Bhandare, NM. Thevasagayam, SRS. Prakki and L. Orbán: Mapping of a major QTL for increased robustness and detection of genome assembly errors in Asian seabass (Lates calcarifer), BMC Genomics 24: 449, 2023
Orbán, L, K. Somfalvi-Tóth, T. Müller, B. Kovács, T. Molnár, I. Szeverényi, Á. Horváth és B. Urbányi: A klímaváltozás lehetséges hatásai tenyésztett halaink élettanára és szaporodására, Állattenyésztés és takarmányozás (közlésre elfogadva), 2023
King, C., Fowler, J.C., Abnizova, I., Sood, R.K., Hall, M.W., Szeverényi, I., Tham, M., Huang, J., Young, S.M., Hall, B.A., Birgitte Lane and PH. Jones: Somatic mutations in facial skin from countries of contrasting skin cancer risk, Nature Genetics, 2023
Fodor, E., J. Okendo, N. Szabo, K. Szabo, D. Czimer, A. Tarjan-Racz, I. Szeverenyi, Bi W.Low, JH. Liew, S. Koren, A. Rhie, L. Orban, A. Miklosi, M. Varga, SM. Burge: The reference genome of the paradise fish (Macropodus opercularis), BioRxiv 2023.08. 10.552018, 2023
Hirth, M., C. Pethő, OM. Szabó, L. Orbán and I. Szeverényi: Revisiting heat-induced masculinization in zebrafish, Poster at EZM2023 (Krakow, Poland) Abstract #327; Abstract Book p. 342, 2023
Szabó, OM., M. Hirth, Gy. Szabó, L. Orbán and I, Szeverényi: Early selection allows for efficient and rapid introgression of a reporter construct into a transparent zebrafish line, Poster at EZM2023 (Krakow, Poland) Abstract #340; Abstract Book p. 354, 2023
Orbán L.: Invited Book Review: Sex Control in Aquaculture by H. Wang, F. Piferrer, S. Chen and Z. G. Shen (Eds). 888pp. Published by John Wiley & Sons, Ltd., Oxford, UK, Journal of Fish Biology 97(2): 590-591, 2020
Orbán L., Bognár A., Havasi M., Szeverényi I., Varga L: Aplication of high-thoughput 'omics' technologies in modern Aquaculture (in Hungarian), Animal Breeding & Feeding 69(3): 317-334, 2020
Várkonyi E., Liptói K., Gócza E., Orbán, L., Urbányi B., Horváth Á.: Long-term, preservation of rare and valuable traits (in Hungarian), Hungarian Agriculture 77(39), pp.30–32, 2022
Shen, XY, YC. Niu, JAV. Uichanco, N. Phua, P. Bhandare, NM. Thevasagayam, SRS. Prakki and L. Orbán: Mapping of a major QTL for increased robustness and detection of genome assembly errors in Asian seabass (Lates calcarifer), BMC Genomics 24: 449, 2023
Orbán, L, K. Somfalvi-Tóth, T. Müller, B. Kovács, T. Molnár, I. Szeverényi, Á. Horváth és B. Urbányi: Potential effects of climate change onto the physiology and reproduction of our cultured fishes (in Hungarian), Animal Breeding & Feeding, 2023. 72. 3, 2023
Fodor, E., J. Okendo, N. Szabo, K. Szabo, D. Czimer, A. Tarjan-Racz, I. Szeverenyi, Bi W.Low, JH. Liew, S. Koren, A. Rhie, L. Orban, A. Miklosi, M. Varga, SM. Burge: The reference genome of the paradise fish (Macropodus opercularis), BioRxiv 2023.08. 10.552018, 2023
Hirth, M., C. Pethő, OM. Szabó, L. Orbán and I. Szeverényi: Revisiting heat-induced masculinization in zebrafish, Poster at 12th European Zebrafish Meeting (EZM2023); Krakow, Poland, Abstract #327; Abstract Book p. 342, 2023
Orbán L.: Invited Book Review: Sex Control in Aquaculture by H. Wang, F. Piferrer, S. Chen and Z. G. Shen (Eds). 888pp. Published by John Wiley & Sons, Ltd., Oxford, UK, Journal of Fish Biology 97(2): 590-591, 2020
Orbán, L., X. Shen, N. Phua and L. Varga: Towards genome-based selection in Asian seabass: What can we learn from food fishes and terrestrial farm animals?, Frontiers in Genetics, 12: 506754, 2021
Koh, A. G.Y. Jing, S. Tao, V. Chaganty, K. See, P. Kathiresan, L. Orbán, A. Mathuru, T. Wohland and C. Winkler: Neurexin2aa deficiency results in axon pathfinding defects and increased anxiety in zebrafish, Human Molecular Genetics 29(23): 3765-3780, 2020
Valdivieso, A., L. Ribas, A. Monleón, L. Orbán and F. Piferrer: Exposure of zebrafish to elevated temperature induces sex ratio shifts and alterations in the testicular epigenome of unexposed offspring, Environmental Research 186: 109601, 2020
Kaldy, J, A. Mozsar, Gy. Fazekas, M. Farkas, D.L. Fazekas, K. Goda, Zs. Gyongy, B. Kovacs, K. Semmens, M. Bercsenyi, M. Molnar and E.P. Varkonyi:: Hybridization of Russian sturgeon (Acipenser gueldenstaedtii, 1833) and American paddlefish (Polyodon spathula, Walbaum 1792) and evaluation of their progeny, Genes 11: 753, 2020
Annus, T., D. Müller, B. Jezsó, Gy. Ullaga, G.M. Harami, L. Orbán, M. Kovács and M. Varga: Bloom syndrome helicase contributes to germ line development and longevity, Cell Death & Disease 13: Art. No. 363, 2022
Várkonyi E., Liptói K., Gócza E., Orbán, L., Urbányi B., Horváth Á.: Ritka és értékes tulajdonságok hosszú távú megőrzése, Magyar Mezőgazdaság 2022. szeptember: 30-32, 2022
Orbán L., Bognár A., Havasi M., Szeverényi I., Varga L: Nagy léptékű ’omikai eljárások’ alkalmazása a modern haltenyésztésben, Állattenyésztés és Takarmányozás 69(3): 317-334, 2020
Koh Angela, Tao Shijie, Jing Goh Yun, Chaganty Vindhya, See Kelvin, Purushothaman Kathiresan, Orbán László, Mathuru Ajay S, Wohland Thorsten, Winkler Christoph: A Neurexin2aa deficiency results in axon pathfinding defects and increased anxiety in zebrafish, HUMAN MOLECULAR GENETICS 29: (23) pp. 3765-3780., 2020
Valdivieso, A., L. Ribas, A. Monleón, L. Orbán and F. Piferrer: Exposure of zebrafish to elevated temperature induces sex ratio shifts and alterations in the testicular epigenome of unexposed offspring, Environmental Research 186: 109601, 2020
Koh, Angela, Tao Shijie, Jing Goh Yun, Chaganty Vindhya, See Kelvin, Purushothaman Kathiresan, Orbán László, Mathuru Ajay S, Wohland Thorsten, Winkler Christoph: A Neurexin2aa deficiency results in axon pathfinding defects and increased anxiety in zebrafish, Human Molecular Genetics 29(23) 3765-3780, 2020
Gyöngyösi, A;,A. Bognár, A, Sz. Nagy: Evaluation of anticoagulant solutions in the flow cytometric analyses of fish blood and larval cells for assessing ploidy and other cell characteristics, Aquaculture Research 53:325–330, 2020
Saju, J.M., M.S. Hossain, W.C. Liew, A. Pradhan, N.M. Thevasagayam, L.S.E. Tan, A. Anand, P-E. Olsson and L. Orbán: Heat shock factor 5 is essential for spermatogenesis in zebrafish, Cell Reports 25(12): 3252-3261.E4, 2018
Orbán, L.: The importance of cell death during gonad transformation in sex changing teleosts, Invited talk at the International Workshop on ‘Autophagy in Model Organisms’; Budapest (Hungary), September 06-08, 2018, 2018
Orbán, L.: Environmental effects on fish: Feed, health and reproduction, Plenary talk at the XIXth International Conference on Risk Factors of Food Chain; Mátrafüred, Hungary, September 26-28, 2018, 2018
Komissarov, A., S. Vij, A. Yurchenko, V. Trifonov, N.M. Thevasagayam, P.S.R. Sridatta, K. Purushothaman, A. Graphodatsky, L. Orbán, I. Kuznetsova:: B chromosomes of the Asian seabass (Lates calcarifer) contribute to genome variations at the level of individuals and populations., Genes 9: 464, 2018
Orbán, L., J.M. Saju, K-W Tzung and WC Liew: Masculinization of zebrafish through partial depletion of primordial germ cells by injecting diluted Morpholino oligonucleotides into embryos., Invited book chapter in the Springer Protocols series on Methods in Molecular Biology (Guest Ed.: Roland Dosch; Series Ed.: J. M Walker) Springer Verlag, Berlin, Germany, 2020
Orbán, L., X. Shen, N. Phua and L. Varga: History of and status report on the Asian seabass (Lates calcarifer) selection program in Singapore, Frontiers in Genetics, submitted (2019), 2020
Ács, É., G. Beliczky, J. Makk, A. Földi, B. Kucska, L. Horváth, Á. Ittzés, Á. Hegyi, B. Urbányi, N.N. Quyen, L. Orbán, M. Havasi and T. Szabó: New observations about fertilization capacity and latency time of sperm inseminated into ovary of African catfish (Clarias gariepinus) as an oviparous modelfish, Aquaculture, under revision, 2020
Orbán, L., X. Shen, N. Phua and L. Varga: History of and status report on the Asian seabass (Lates calcarifer) selection program in Singapore, Frontiers in Genetics, under review (2020), 2020
Müller, T, É. Ács, G. Beliczky, J. Makk, A. Földi, B. Kucska, L. Horváth, Á. Ittzés, Á. Hegyi, B. Urbányi, N.N. Quyen, L. Orbán, M. Havasi and T. Szabó: New observations about fertilization capacity and latency time of sperm inseminated into ovary of African catfish (Clarias gariepinus) as an oviparous modelfish, Aquaculture 522: 735109, 2020
Miyake, S., M. Soh, M.N. Azman, S.Y. Ngoh, L. Orbán and H. Seedorf: Insights into the microbiome of farmed Asian sea bass (Lates calcarifer) with tenacibaculosis symptoms and description of Tenacibaculum singaporense sp. nov., Antonie von Leuwenhoek Journal of Microbiology 113: 737-752, 2020
Bognár, A., M.H. Borkhanuddin, S. Nagase, K. Molnár, Cs. Székely, B. Sellyei, I. Szeverényi and L. Orbán: Gill biopsy-based sorting of European catfish (Silurus glanis L., 1758) allows for reduced variation of parasite counts within experimental groups., Poster presentation at Aquaculture Europe 2023 (Vienna, Austria); September 18-21, 2023
Beliczky, G., Havasi, M., Merth, J., Bognár, A., Sellyei, B., Kovács, Gy., Horváth, Á., Szeverényi, I., Orbán, L., Bercsényi, M.: A Hungarian selection program of European catfish (Silurus glanis L. 1758) supported by genetic platforms and technological improvements., Poster presentation at Aquaculture Europe 2023 (Vienna, Austria); Abstract Book p. 118, 2023
Szabó, O.M.: Introducing a reporter gene with sex-specific expression pattern into transparent zebrafish using an early selection method (in Hungarian), Oral presentation at the National Science Competition of University Students (OTDK); Kaposvár, Hungary, April 4-6., 2023
Szabó, O.M., M. Hirth, Gy. Szabó, L. Orbán and I. Szeverényi: Early selection allows for efficient and rapid introgression of a reporter construct into a partially transparent zebrafish line, Flash talk at the 12th European Zebrafish Meeting (EZM2023); Krakow, Poland, July 9-13, 2023
Szabó, O.M., M. Hirth, Gy. Szabó, L. Orbán and I. Szeverényi: Early selection for efficient introduction of a reporter gene with sex-specific expression pattern into transparent zebrafish (in Hungarian), Invited oral presentation at the 28th Conference of the Society of Reproduction Biologists; Szarvas, Hungary, November 10-11., 2023
Orbán, L.: Zebrafish as a model for natural sex reversal of teleosts., Keynote presentation at the 5th Conference on Cell, Developmental and Germ Cell Biology (SFB); Budapest, Hungary, October 27., 2023
Hartl. M.: Analysis of the status of Hévíz dwarf carp (Cyprinus carpio carpio morpha hungaricus) using the tools of classical aquaculture (in Hungarian)., Oral presentation at the Regional Science Competition of University Students (TDK); Keszthely, Hungary, November, 22., 2023
Pethő, C.: Revisiting heat-induced masculinization in zebrafish model., Oral presentation at the National Science Competition of University Students (OTDK); Kaposvár, Hungary, April 4-6, 2023
Pethő, C.: Revisiting heat-induced masculinization in zebrafish model (in Hungarian), Oral presentation at the Regional Science Competition of University Students (TDK); Keszthely, Hungary, November, 23., 2022
Hirth, M., Pethő, C., Szabó, O.M., Orbán, L., Szeverényi I.: Analysis of heat-induced sex reversal in zebrafish (in Hungarian), Poster presentation at the 5th Conference on Cell, Developmental and Germ Cell Biology (SFB); Budapest, Hungary, October 27., 2023




 

Events of the project

  
2022-08-29 12:52:06
Résztvevők változása
2022-01-11 16:51:59
Résztvevők változása
2020-04-16 17:33:11
Résztvevők változása
2019-08-29 12:14:58
Résztvevők változása



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