Molekuláris Biológia (Orvosi és Biológiai Tudományok Kollégiuma)
90 %
Ortelius tudományág: Molekuláris biológia
Immunológia (Orvosi és Biológiai Tudományok Kollégiuma)
10 %
Ortelius tudományág: Immunológia
zsűri
Immun-, Tumor- és Mikrobiológia
Kutatóhely
ÁOK Orvosi Vegytani Intézet (Debreceni Egyetem)
projekt kezdete
2016-02-01
projekt vége
2019-01-31
aktuális összeg (MFt)
22.704
FTE (kutatóév egyenérték)
3.00
állapot
aktív projekt
Zárójelentés
kutatási eredmények (magyarul)
In this work we have performed and controlled Chromatin immunoprecipitation experiments using PPARg and RXRa antibodies as well as expression experiments aiming to subsequently perform ChIP- and RNA-Seq experiments. Among the known targets of PPARg, several of them were found to be enriched in our ChIP experiments, but unfortunately none of them gave a strong signal in the ChIP-seq experiment, neither for PPARg nor for RXRa. The strongest peaks in our ChIP-seq experiments correspond to genes associated with diverse functions but they are difficult to be discussed in the context of the M2 phenotype. We have recently modified the immunoprecitipation protocol aiming to perform ChIP-seq experiments providing us with reliable data concerning the targets of PPARg and RXRa in monocyte-derived machrophages.
kutatási eredmények (angolul)
In this work we have performed and controlled Chromatin immunoprecipitation experiments using PPARg and RXRa antibodies as well as expression experiments aiming to subsequently perform ChIP- and RNA-Seq experiments. Among the known targets of PPARg, several of them were found to be enriched in our ChIP experiments, but unfortunately none of them gave a strong signal in the ChIP-seq experiment, neither for PPARg nor for RXRa. The strongest peaks in our ChIP-seq experiments correspond to genes associated with diverse functions but they are difficult to be discussed in the context of the M2 phenotype. We have recently modified the immunoprecitipation protocol aiming to perform ChIP-seq experiments providing us with reliable data concerning the targets of PPARg and RXRa in monocyte-derived machrophages.
A kutatás helye megváltozott. Korábbi kutatóhely: ÁOK Biokémiai és Molekuláris Biológiai Intézet (Debreceni Egyetem), Új kutatóhely: ÁOK Orvosi Vegytani Intézet (Debreceni Egyetem).
