Molecular biology (Council of Medical and Biological Sciences)
90 %
Ortelius classification: Molecular biology
Immunology (Council of Medical and Biological Sciences)
10 %
Ortelius classification: Immunology
Panel
Immunity, Cancer and Microbiology
Department or equivalent
Department of Medical Chemistry (University of Debrecen)
Starting date
2016-02-01
Closing date
2019-01-31
Funding (in million HUF)
22.704
FTE (full time equivalent)
3.00
state
running project
Final report
Results in Hungarian
In this work we have performed and controlled Chromatin immunoprecipitation experiments using PPARg and RXRa antibodies as well as expression experiments aiming to subsequently perform ChIP- and RNA-Seq experiments. Among the known targets of PPARg, several of them were found to be enriched in our ChIP experiments, but unfortunately none of them gave a strong signal in the ChIP-seq experiment, neither for PPARg nor for RXRa. The strongest peaks in our ChIP-seq experiments correspond to genes associated with diverse functions but they are difficult to be discussed in the context of the M2 phenotype. We have recently modified the immunoprecitipation protocol aiming to perform ChIP-seq experiments providing us with reliable data concerning the targets of PPARg and RXRa in monocyte-derived machrophages.
Results in English
In this work we have performed and controlled Chromatin immunoprecipitation experiments using PPARg and RXRa antibodies as well as expression experiments aiming to subsequently perform ChIP- and RNA-Seq experiments. Among the known targets of PPARg, several of them were found to be enriched in our ChIP experiments, but unfortunately none of them gave a strong signal in the ChIP-seq experiment, neither for PPARg nor for RXRa. The strongest peaks in our ChIP-seq experiments correspond to genes associated with diverse functions but they are difficult to be discussed in the context of the M2 phenotype. We have recently modified the immunoprecitipation protocol aiming to perform ChIP-seq experiments providing us with reliable data concerning the targets of PPARg and RXRa in monocyte-derived machrophages.
A kutatás helye megváltozott. Korábbi kutatóhely: ÁOK Biokémiai és Molekuláris Biológiai Intézet (Debreceni Egyetem), Új kutatóhely: ÁOK Orvosi Vegytani Intézet (Debreceni Egyetem).
