Molecular analysis of phytochrome induced signal transduction in Arabidopsis thaliana  Page description

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Details of project

 
Identifier
46710
Type K
Principal investigator Nagy, Ferenc
Title in Hungarian A fitokróm fotoreceptorok indukálta jelátviteli lánc molekuláris vizsgálata Arabidopsis thalianában
Title in English Molecular analysis of phytochrome induced signal transduction in Arabidopsis thaliana
Panel Molecular and Structural Biology and Biochemistry
Department or equivalent Institute of Plant Biology (Biological Research Centre Szeged)
Participants Ádám, Éva
Fejes, Erzsébet
Kevei, Éva
Medzihradszky, Mátyás
Valkai, Ildikó
Starting date 2004-01-01
Closing date 2006-12-31
Funding (in million HUF) 9.228
FTE (full time equivalent) 0.00
state closed project





 

Final report

 
Results in Hungarian
Bebizonyítottuk, hogy a fitokróm-A sejtmagba történő transzlokációjában és sejtmagi akkumulációjában, tehát a fitokróm-A szabályozta jelátviteli lánc aktiválásában az FHY1 fehérje meghatározó szerepet játszik. Kimutattuk, hogy a fitokróm-A autofoszforilációja negatívan szabályozza a jelátviteli lánc aktivitását és azonosítottunk egy olyan foszfatázt, ami specifikusan a fitokróm-A fotoreceptor defoszforilációjáért felelős. Bebizonyítottuk, hogy a PIF3 fehérje nem pozitív, hanem negatív regulátora a fotomorfogenezisnek, a PIF3 fehérje nem szükséges a cirkadián óra működéséhez, továbbá, hogy fény hatására a PIF3 fehérje gyorsan degradálódik és hogy a fitokróm- A-B és D fotoreceptorok együttesen szabályozzák a PIF3 transzkripciós faktor fény indukálta degradációját.
Results in English
We have provided compelling evidence that nuclear translocation and accumulation of phytochrome-A requires the FHY1 protein and that FHY1 interacts with the phyA molecule in a conformation dependent fashion and this interaction is mediated by the very N-terminal domain of the photoreceptor. We demonstrated that PIF3 transcription factor negatively regulates phytochrome controlled photomorphogenesis, its function is not required for light dependent entrainment of the plant circadian clock and that phytochrome-A in harmony with phytochrome-B and D regulates light induced degradation of PIF3. Moreover, we showed that interaction of phytochrome-A with various components of the phytochrome-A dependent signalling cascade is negatively regulated by the autophosphorylation of the photoreceptor and that dephosphorylation of the photoreceptor is mediated by a specific phosphatase.
Full text http://real.mtak.hu/1547/
Decision
Yes





 

List of publications

 
Bauer, D., Viczián, A., Kircher, S., Nobis, T., Kunkel, T., Panigrahi, K., Ádám, É., Fejes, E., Schafer, E., Nagy, F.: COP1 and multiple photoreceptors control degradation of the negative regulator PIF3, a transcription factor, required for light signalling in arabidopsis, Plant Cell 16, 1433-1445, 2004
Ulm, R., Baumann, A., Oravecz, A., Mate, Z., Ádám, É., Oakeley, J., Schafer, E., Nagy, F.: Genome-wide analysis of gene expression reveals HY5 function in the UV-B response of Arabidopsis, Proc. Natl. Acad. Sci. USA 101, 1379-1402, 2004
Turck, F., Zilbermann, F., Kozma, C.S., Thomas, G., Nagy, F.: Phytohormones participate in an S6 kinase signal transduction pathway in Arabidopsis thaliana, Plant Physiol. 134, 1-9, 2004
Kim, J.I., Shen, Y., Han, Y.J., Park, J.E., Kirchenbauer, D., Soh, M.S., Nagy, F., Schafer, E., Song, P.S.: Phytochrome phosphorylation modulates light signaling by influencing the protein-protein interaction, Plant Cell 16, 2629-2640, 2004
Edwards, K.D., Lynn, J.R., Gyula, P., Nagy, F., Millar, A.J.: Natural Allelic Variation in the Temperature Compensation Mechanisms of the Arabidopsis thaliana Circadian Clock, Genetics 170, 387-400, 2005
Fernandez, A.P., Gil, P., Valkai, I., Nagy, F., Schäfer, E.: Analysis of the Function of the Photoreceptors Phytochrome-B and Phytochrome-D in Nicotiana plumbaginifolia and Arabidopsis thaliana, Plant Cell Physiol. 46, 790-796, 2005
Ryu, J.S., Kim, J.I., Kunkel, T., Kim, B.C., Cho, D.S., Hong, S.H., Kim, S.H., Fernandez, A.P., Kim, Y., Alonso, J.M., Ecker, J.R., Nagy, F., Lim, P.O., Song, P.S., Schäfer, E., Nam, H.G.: Phytochrome-specific type 5 phosphatase controls light signal flux by enhancing phytochrome stability and affinity for a signal transducer, Cell 120, 395-406, 2005
Dodd, A.N., Salathia, N., Hall, A., Kevei, É., Tóth, R., Nagy, F., Hibberd, J.M., Millar, A.J., Webb, A.A.: Plant circadian clocks increase photosynthesis, growth, survival, and competitive advantage, Science 309, 630-633, 2005
Ulm, R., Nagy, F.: Signalling and gene regulation in response to ultraviolet light, Curr. Opin. Plant Biol. 8, 477-482, 2005
Viczián, A., Kircher, S., Fejes, E., Millar, A.J., Schäfer, E., Kozma-Bognár, L., Nagy, F.: Functional Characterization of Phytochrome Interacting Factor 3 for the Arabidopsis thaliana Circadian Clockwork, Plant Cell Physiol. 46, 1591-1602, 2005
Hiltbrunner, A., Viczián, A., Bury, E., Tscheuschler, A., Kircher, S., Toth, R., Honsberger, A., Nagy, F., Fankhauser, C., Schäfer, E.: Nuclear Accumulation of the Phytochrome a Photoreceptor Requires FHY1, Curr. Biol. 15, 2125-2130, 2005




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